[Webinar] Proteomic analysis of adult and aged mouse hematopoietic stem cells and their progenitors

4th Thought Leadership Webinar from PreOmics

We are excited to announce our speaker Dr Balyn Zaro from the University of California, San Francisco presenting on: "Proteomic analysis of adult and aged mouse hematopoietic stem cells and their progenitors".

Abstract:

The balance of hematopoietic stem cell (HSC) self-renewal and differentiation is critical for a healthy blood supply; imbalances underlie hematological diseases. The importance of HSCs and their progenitors have led to their extensive characterization at genomic and transcriptomic levels. However, the proteomics of hematopoiesis remains incompletely understood. Here we report a proteomics resource from mass spectrometry of mouse adult and aged HSCs, multipotent progenitors and oligopotent progenitors; 12 cell types in total. We validated differential protein levels, including confirmation that Dnmt3a protein levels are undetected in adult HSCs until forced into cycle. Additionally, through integrating proteomics and RNA sequencing datasets, we identified a subset of genes with apparent post-transcriptional repression in adult HSCs. In summary, we report proteomic coverage of adult and aged HSCs and progenitors, with broader implications for understanding mechanisms for stem cell maintenance, niche interactions and fate determination.

Dr Balyn Zaro – Assistant Professor, Department of Pharmaceutical Chemistry, University of California, San Francisco, USA

Balyn Zaro, PhD, attended the University of Southern California for her PhD studies in chemical biology as the first graduate student in the laboratory of Matthew Pratt, PhD. Her research focused on developing metabolic bioorthogonal chemical reporters to identify and to characterize post-translational modifications of proteins, including glycosylation, acetylation, and ubiquitination. For her postdoctoral studies, she worked in the laboratory of Ben Cravatt, PhD, at Scripps Research Institute in La Jolla, California. There she investigated the metabolism of covalent small molecules using activity-based protein profiling and identified the mechanisms of action of the multiple sclerosis drug Tecfidera® (dimethyl fumarate). She gained additional training with Irving Weissman, MD, at Stanford University School of Medicine in the fields of innate immunity and hematopoiesis before her arrival at UCSF in September 2019.

This webinar took place on October 12, 2021Fill in this form to access the recording

Thought Leadership Webinar Series

Proteomic analysis of adult and aged mouse hematopoietic stem cells and their progenitors

Agenda and Speakers